GB 4789.38-2012: Translated English of Chinese Standard. GB4789.38-2012: National Food Safety Standard - Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli
https://www.chinesestandard.net, Dec 2, 2017 - Technology & Engineering - 16 pages
The standard specifies the counting method of Escherichia coli in food. This standard is applicable to the counting of Escherichia coli in food. Among them, plate count method for Escherichia coli (Method II) is not applicable to shellfish products.
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Culture Medium and Reagent
Plate Count Method for Escherichia Coli Method II
Appendix A Culture Medium and Reagent
Appendix B Most Probable Number MPN Search List of Escherichia Coli
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1:10 sample homogeneous 1min 225mL phosphate buffer 5.0g Dipotassium hydrogen adjust pH Appendix aqueous solution autoclave for 15min bile salt 1.5g biochemical assessment Buffer glucose peptone colony number compositions in distilled continuous dilutability Counting Escherichia Coli Crystal violet cultivate for 18h~24h diluent Dipotassium hydrogen phosphate Distilled water 1000.0mL EC broth tube enteroaerogen Eosin methylene blue Escherichia Coli Method ethanol fermentation test filled with 225mL glucose peptone water Gram negative Gram staining hydrochloric acid hydrogen phosphate K2HPO4 IMViC biochemical test inoculating loop iodine Kovacs indole reagent LST broth tube Medium and Reagent method for Escherichia Monopotassium phosphate MPN for Counting Neutral red No.3 bile salt Nutrient agar slant Paradime Pentanol plate count method potassium hydroxide Preparation Dissolve prepare 1:10 sample Safranin sample homogeneous solution Sodium chloride 5.0g sodium hydroxide Sub-add Sub-inject sucker head Test method Inoculate Test Procedures test tube Tryptone or trypticase V-P tests Violet red bile VRBA-MUG water and adjust