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General Experimental Design
Cytotoxic T Cells in Reticuloendotheliosis
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allogeneic antigens birds bursa Calnek cell-mediated cytotoxicity Cell-mediated immune responses characterized Chen chicken T cell chromium release assay clones complement CTL activity CTL and NK CTL responses cytolysis cytolytic cytotoxic activity cytotoxic T cells cytotoxic T lymphocytes days-post-inoculation demonstrated depletion E:T ratio effector cells embryonally bursectomized evaluated fluorescent functionally haplotypes herpesvirus hybridoma identified immunoglobulin Immunohistochemical staining Immunol inoculated Lillihoj LM Hahn lymphoblastoid lymphoid lyse lysis macrophages major histocompatibility complex mammalian Marek's disease virus McCubbin and Schierman MHC class MHC-restricted cytotoxicity monoclonal antibodies natural killer NK activity NK cells nylon wool oncogenic P-2a chickens previously described proliferation proteins resuspended reticuloendotheliosis virus REV-infected P-2a REV-inoculated REV-transformed cell lines serum specific release spleen cells splenic lymphocytes stimulation supernatant suppressor syngeneic target antigens techniques thymocytes thymus tissue culture Toivanen tumor cell lines uninfected vaccine virus-infected vitro vitro cultivation vivo washed Weinstock and Schat