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70 per cent absolute alcohol acetic acid acidulated added alcoholic solution alum alum-carmine ammonia Anat anilin anilin-blue aqueous solution Arch bichromate blue boiling borax-carmine Canada balsam carminic acid celloidin cells chloroform chromic acid clear clove oil collodion colouring matter concentrated solution connective tissue dammar dehydrated dilute dissolved distilled water double stain drop employed eosin ether evaporation filter fixing agent fluid formic formula fresh gelatin glycerin gold chloride grammes haematoxylin hardened Ibid imbedding immersion injected Injection-mass iodine-green Journ kreasote liquid maceration mass medium melted method microtome minutes mixture mount in balsam nitrate nitric nuclear stain nuclei objects obtained oil of cloves osmic acid ounces oxalic acid paraffin picric acid picro-carmine potash precipitate preparations preserved protoplasm quantity Ranvier reagent recommended removed safranin salt saturated solution sections slide soaked solu soluble tion treated turpentine twenty-four hours violet warm washed watch-glass water-bath whilst yellow Zool