The Microtomist's Formulary and GuideThe art of making microscope slides. Methods and formulas used in making microscope slides. |
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Page 455
Peter Gray. 22.8 Beyard 1930 → 4285a , 7 : 264 REAGENTS REQUIRED : A. 1 % acid fuchsin ; B. 0.8 % bromine METHOD : [ sections ] → water → A , 30 secs . → rinse - B , 4-5 ... REAGENTS REQUIRED DS 22.8 455 DYE STAINS OF SPECIAL APPLICATION.
Peter Gray. 22.8 Beyard 1930 → 4285a , 7 : 264 REAGENTS REQUIRED : A. 1 % acid fuchsin ; B. 0.8 % bromine METHOD : [ sections ] → water → A , 30 secs . → rinse - B , 4-5 ... REAGENTS REQUIRED DS 22.8 455 DYE STAINS OF SPECIAL APPLICATION.
Page 535
... REAGENTS REQUIRED : A. 0.5 % gold chloride ; B. 20 % formic acid METHOD : [ pieces of fresh tissue , left without preservative at 4 ° C . for 48 hrs . ] dark , 1⁄2 to 34 hrs . → rinse → B , till nerves visible glycerol , several ...
... REAGENTS REQUIRED : A. 0.5 % gold chloride ; B. 20 % formic acid METHOD : [ pieces of fresh tissue , left without preservative at 4 ° C . for 48 hrs . ] dark , 1⁄2 to 34 hrs . → rinse → B , till nerves visible glycerol , several ...
Page 561
... reagents 1930 623 , 14 : 394 REAGENTS REQUIRED : A. 1 % silver nitrate at pH 4.4 ; B. AMS 21.1 Farrier and Warthin 1930 ; C. 5 % sodium thiosulfate METHOD : [ sections of formaldehyde - fixed material ] → A , 30 mins . , 37 ° C . , in ...
... reagents 1930 623 , 14 : 394 REAGENTS REQUIRED : A. 1 % silver nitrate at pH 4.4 ; B. AMS 21.1 Farrier and Warthin 1930 ; C. 5 % sodium thiosulfate METHOD : [ sections of formaldehyde - fixed material ] → A , 30 mins . , 37 ° C . , in ...
Contents
INTRODUCTION | 1 |
FOREWORD TO PART I | 7 |
PRESERVATIVES REFERENCED AS P 175 | 20 |
Copyright | |
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absolute alcohol acetic acid acetone acid fuchsin ammonia aniline balsam block Cajal carmine Castro cell celloidin cement Chapter chloroform chromic acid circ clove oil coverslip dehydrated differentiated diluted dish Dissolve distilled water drop embedding embryo eosin ferric alum Filter fixed material fluid formaldehyde formaldehyde-fixed material FORMULA fresh frozen sections Gatenby gelatin glass glycerol gold chloride hardened hematoxylin hydrochloric acid hydroxide injection knife Langeron Mallory medium mercuric chloride methanol methyl methylene blue microscope microtome mins mixture mordant mountant mounted needle nitric NOTE nuclei object orange G osmic acid paraffin paraffin sections phenol picric acid placed potassium dichromate PREPARATION quick rinse REAGENTS REQUIRED removed Río-Hortega safranin Schmorl sections of F silver nitrate slide sodium thiosulfate specimen stain STOCK SOLUTIONS sulfate surface technique tion tissues usual reagents RECOMMENDED wash water 50 Weigert wholemounts xylene