The Microtomist's Formulary and GuideThe art of making microscope slides. Methods and formulas used in making microscope slides. |
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Page 78
... tion , and the only question to be settled is whether or not the finished slide should show muscle cells in an expanded condi- tion , in which case the hydra will have to be narcotized before fixation , or whether it will be sufficient ...
... tion , and the only question to be settled is whether or not the finished slide should show muscle cells in an expanded condi- tion , in which case the hydra will have to be narcotized before fixation , or whether it will be sufficient ...
Page 79
... tion . The tube is now shaken gently until such time as each hydra has dissociated into its constituent cells . If this does not take place after shaking for a few minutes , it is necessary to withdraw the alum solu- tion , replace it ...
... tion . The tube is now shaken gently until such time as each hydra has dissociated into its constituent cells . If this does not take place after shaking for a few minutes , it is necessary to withdraw the alum solu- tion , replace it ...
Page 275
... tion has been seen . If a low - power examination of the sec- tion shows the nuclei to be standing out clearly , the entire tray should be removed to distilled water , because from this time on differentiation is very rapid and each ...
... tion has been seen . If a low - power examination of the sec- tion shows the nuclei to be standing out clearly , the entire tray should be removed to distilled water , because from this time on differentiation is very rapid and each ...
Contents
INTRODUCTION | 1 |
FOREWORD TO PART I | 7 |
PRESERVATIVES REFERENCED AS P 175 | 20 |
Copyright | |
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absolute alcohol acetic acid acetone acid fuchsin ammonia aniline balsam block Cajal carmine Castro cell celloidin cement Chapter chloroform chromic acid circ clove oil coverslip dehydrated differentiated diluted dish Dissolve distilled water drop embedding embryo eosin ferric alum Filter fixed material fluid formaldehyde formaldehyde-fixed material FORMULA fresh frozen sections Gatenby gelatin glass glycerol gold chloride hardened hematoxylin hydrochloric acid hydroxide injection knife Langeron Mallory medium mercuric chloride methanol methyl methylene blue microscope microtome mins mixture mordant mountant mounted needle nitric NOTE nuclei object orange G osmic acid paraffin paraffin sections phenol picric acid placed potassium dichromate PREPARATION quick rinse REAGENTS REQUIRED removed Río-Hortega safranin Schmorl sections of F silver nitrate slide sodium thiosulfate specimen stain STOCK SOLUTIONS sulfate surface technique tion tissues usual reagents RECOMMENDED wash water 50 Weigert wholemounts xylene