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24 hours 30 minutes 40 percent formaldehyde 70 percent alcohol 95 percent absolute alcohol Absolute ethyl alcohol acetone block Blue bone calcium cell celloidin changes of distilled clearing agent color cytoplasm Dehydrate Differentiate DISADVANTAGES distilled water dyes eosin ethyl alcohol filter FIXATION Ten percent fixative formaldehyde frozen sections g Distilled Glacial acetic acid glycogen granules harden hematoxylin hone hydrochloric acid impregnated iodine knife mercuric chloride microscope microtome Mitochondria ml Dissolve ml Distilled water mold Mordant neutral nuclear staining osmium tetroxide paraffin wax percent aqueous percent formalin percent potassium percent silver nitrate percent sodium thiosulphate percent solution picric acid pigment precipitate Preserved proteins ratchet wheel reagents removed RESULTS Nuclei Rinse in distilled Rinse in tap routine running tap water saturated Sections to distilled Sections to water shrinkage slide soluble specimen strop tap water technique thickness Wash in distilled Wash in running Weigert's iron hematoxylin xylene