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acetone additional apparatus apparent solubility average solubility CD CD CD CD CM cd H CM CD CM CM CM cn cn condensed cosolvent degree of n-3 determined docosahexaenoic acid effect entrainer EPA+DHA experimental extraction conditions extraction of menhaden extraction pressure extraction system extraction temperature extraction trial extraction vessel fatty acid composition fatty acid sample fish oils fractions free fatty acids gas chromatography glycerides H CM hexane increased lipid liquid menhaden fatty acids menhaden methyl esters methanol mixture molecular weight n-3 components n-3 concentration n-3 enrichment n-3 fatty acids obtained optimal Oven Phase 1 experiments Phase 2 extraction POT sample process conditions reflux column reflux flow reflux pump reflux temperature rH rH sample form sample recovery sample vessel separation vessel solute solvent flow species sterols supercritical carbon dioxide supercritical extraction supercritical solvent systematic series Table TEMP temperature and pressure triglycerides vitamin Zosel