Chemical Methods in Prokaryotic SystematicsMichael Goodfellow, Anthony G. O'Donnell The use of chemical techniques is having a profound effect on approaches to the characterization, classification and identification of prokaryotes, i.e. archaea and bacteria. This book provides a comprehensive, up-to-date treatment of such methods used in prokaryotic systematics and is written by leading specialists in this field. Designed as a laboratory manual, this volume describes the latest applications to prokaryote systematics of powerful techniques such as protein electrophoresis, pyrolysis mass spectrometry and rapid enzyme testing. The extraction, purification and characterization of specific components such as fatty acids, lipid A, peptidoglycan, pigments (carotenoids and bacteriochlorophylls) and isoprenoid quinones are explained. The value of these methods in the circumscription and identification of archaea and bacteria is demonstrated. The book is aimed at microbiologists and microbial technologists interested in characterizing and identifying bacteria. |
Contents
Chemosystematics Current State and Future Prospects | 1 |
References | 16 |
Analysis of Cell Wall Constituents of Grampositive | 63 |
Copyright | |
21 other sections not shown
Common terms and phrases
absorption Academic Press acetate aerobic amino analysis archaea archaebacteria bacteria bacteriochlorophyll Bacteriol bacterium Bchl Biochem Biophys biosynthesis Brockmann Campylobacter carbon carotenoids cell wall Chem chemical Chlorobium chloroform chlorophyll chromatography coli Collins column complex lipids components composition compounds containing cytochrome derivatives detection diether double bonds electrophoretic eluted enzymes esterifying esters ether extraction fatty acids Figure Gambacorta GDGTs genus glycerol glycolipids Goodfellow haem halophilic hopanoids HPLC hydrolysis identification isolated isoprenoid isoprenoid quinones mass spectrometry membrane menaquinones methanogenic methanol method methyl microbial Microbiol Minnikin mixture molecular molecules organisms oxidase oxidation patterns peak peptidoglycan phospholipids photosynthetic phototrophic pigments plates polar lipids procedure profiles prokaryotes protein purified purple bacteria pyrolysis quinones Rosa S-layer sample saturated separation side-chain silica solution solvent species spectra spectrum sterols strains structure substrates sugars Sulfolobus taxa taxonomic techniques temperature tetraethers tion ubiquinones wavelength Woese