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Acad Sci USA activity amino acids anti-HIV antibody-dependent enhancement antigen antiviral assay binding buffer CD4+ cell lines cellular clinical clone concentrations culture cytopathic cytotoxic detected dilution disease effect ELISA enhancement envelope glycoprotein epitope expression fibroblasts Gallo RC gene glycoprotein HIV infection HIV-1 isolates HIV-1 RT HSRV HTLV-I HTLV-IIIB human immunodeficiency virus Human Retroviruses hybridization IDR peptide immune immunoassay immunodeficiency virus type Immunol incubated infected cells inhibition lane liposomes lymphocytes lymphotropic MAbs macaques macrophages Mary Ann Liebert molecular monoclonal antibody monocytes mononuclear Natl Acad Sci neutralizing antibodies p24 antigen patients peptide peripheral blood polypeptides Proc Natl Acad production protein purified reactivity receptor recombinant region reverse transcriptase rhesus macaques samples sCD4 Science sequence sera seropositive serum specific strain studies supernatant syncytium T-cell target cells tested titers transcription transfected uninfected vaccine viral Virol virus infection viruses vitro Western blot Wong-Staal