Guide to Yeast Genetics and Molecular BiologyChristine Guthrie Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein |
Contents
Cloning and Recombinant DNA | 167 |
Making Mutants | 271 |
Biochemistry of Gene Expression | 371 |
Cell Biology | 563 |
Fission Yeast | 793 |
Appendix | 825 |
| 865 | |
| 905 | |
Other editions - View all
Guide to Yeast Genetics and Molecular Biology, Part 1 Christine Guthrie,Gerald R. Fink No preview available - 1991 |
Common terms and phrases
Acad acetate acid agar allele analysis antibodies assay buffer Cell Biol Cell Cambridge centrifugation centromere cerevisiae chromosome chromosome fragment cloned Cold Spring Harbor coli colonies concentration containing culture described diluted diploid E. W. Jones EcoRI EDTA electrophoresis enzyme ethanol extract filter fluorescence fraction fusion galactose gene genetic genome glucose growth haploid HindIII immunofluorescence incubation insert isolated labeling LEU2 lysis mapping marker MATa MATo medium meiosis meiotic membrane method mg/ml microscope mitochondria mitochondrial molecular mRNA mutagenesis mutagenized mutants Natl nuclear nuclei o-factor pellet pg/ml phenotype plasmid pombe prepared Proc procedure protease protein protocol purified recombination replica plated replication Resuspend ribosomal room temperature Saccharomyces Saccharomyces cerevisiae sample segregation selection sequences solution sorbitol specific spheroplasts spores sporulation supernatant tion transcription transformation Tris-HCl tube URA3 vacuole vector vitro volume wash wild-type yeast yeast cells yeast strains YEPD