Molecular and Cell Biology |
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Page 83
... specific activity under the same conditions ) , a correction for this loss was applied to the specific activity of RNA to give the final figures used . but to require the mediation of enzymatic components of the pH 5 enzyme fraction ...
... specific activity under the same conditions ) , a correction for this loss was applied to the specific activity of RNA to give the final figures used . but to require the mediation of enzymatic components of the pH 5 enzyme fraction ...
Page 93
... specific with regard to the amino acid which it links to RNA . Table 3 , experiment 1 , shows that the purified ... activity 6.05 X 10 c.p.m per μmole ) ; 0.2 μmole of C14 - DL - phenylalanine ( specific activity 1.1 X 10 c.p.m. per ...
... specific with regard to the amino acid which it links to RNA . Table 3 , experiment 1 , shows that the purified ... activity 6.05 X 10 c.p.m per μmole ) ; 0.2 μmole of C14 - DL - phenylalanine ( specific activity 1.1 X 10 c.p.m. per ...
Page 94
... Activity at Various Stages of Purification * Specific activity ( Units per Mg . of Protein ) Fraction Crude extract A - 2 AS - 1 AS - 2b Cy cluate 0.20 5.4 9.2 15 19 Activity At ( Units per MI . ) Activity B ( Units per MI . ) A / B 4.5 ...
... Activity at Various Stages of Purification * Specific activity ( Units per Mg . of Protein ) Fraction Crude extract A - 2 AS - 1 AS - 2b Cy cluate 0.20 5.4 9.2 15 19 Activity At ( Units per MI . ) Activity B ( Units per MI . ) A / B 4.5 ...
Contents
Watson and F H C Crick | 3 |
Matthew Meselson and Franklin W Stahl | 14 |
Maurice J Bessman I R Lehman Ernest S Simms and Arthur Korn | 28 |
Copyright | |
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acetate addition adenosine triphosphate adenyl amino acid amino acid appear ascorbate bacterial band bases basophil Biochem Biol Biophys buffer carbon dioxide carbon dioxide fixation cells cent centrifugation chain Chem chemical chlorophyll chlorophyll molecules chloroplasts chromatids chromosomes codons coli components concentration containing cytoplasm dATP dCTP deoxynucleoside triphosphates deoxynucleotides described dGTP DNA molecule DNase dTTP electron endoplasmic reticulum enzymatic enzyme enzyme fraction Euglena experiment extract glucose gradient hexokinase incorporation incubation infection inorganic phosphate isolated chloroplasts labeled leucine leucine-C¹¹ magnesium membrane messenger RNA micrographs microsomes minutes molecular weight N¹¹ NaCl norite nucleic acid nucleotide omitted optical density oxygen Palade particles perchloric acid pH 5 enzyme pH 5 RNA phage photosynthetic phosphorylation polymerase precipitate prepared present protein synthesis radioactivity reaction mixture ribosomes RNA-amino acid sequence shown small granules solution specific activity sRNA structure subunits supernatant Table template thymidine thymus DNA tion transfer trinucleotides