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1ars agar mediums allow air flow bacteria become contaminated bottom third city tap water cooker cooking cotton cover Crack the lid cubensis culture Dextrose dikaryotic mycelium dishes or slants filter paper fresh cow manure germinates gloves grain jars grain medium grams agar grow mushrooms heap hypha inch inoculation box inoculum inside keep lamp layer Leave the lid lid cracked open method micron filter mixed mixture moist moisture monokaryotic mycelium mushroom compost mushroom grows mushroom mycelium mushroom spores mushrooms 10 grams mycelium clump mycelium growth mycelium takes mycelium will grow MYCOLOGY needed non-sterile medium nutrients oxygen flow petri dish place in sterile Plug plunger potato water pressure cooker Put the needle ready remove sealing shake the jar small jar snag snores solution somatogamy species spore print spread the mycelium sterile area sterile end sterile water strain straw syringe tape temperature Tighten the lid transfer tube Universal veil vermiculite