Developmental Biology Protocols
Rocky S. Tuan, Cecilia W. Lo
Springer Science & Business Media, Jan 21, 2000 - Science - 522 pages
Drawing on state-of-the-art cellular and molecular techniques as well as new and sophisticated imaging and information technologies, this comprehensive, three-volume collection of cutting-edge protocols provides readily reproducible methods for studying and analyzing the events of embryonic development. Volume 1 (ISBN: 089603-574-3) contains techniques for establishing and characterizing several widely used experimental model systems, for the study of developmental patterns and morphogenesis, and for the examination of embryo structure and function. There are also step-by-step methods for the analaysis of cell lineage, the production and use of chimeras, and the experimental and molecular manipulation of embryos, including the application of viral vectors. Volume 2 (ISBN: 0-89603-575-1) describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 (ISBN: 0-89603-576-X) introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines.
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acid aliquots amount analysis antigen antisense approx assay Biol buffer cadherins catenins cellular centrifuge chick embryo clones collagen concentration containing coverslip culture dish culture medium detection Developmental Biology differentiation digestion diluted dissecting Drosophila ectoderm EDTA eggs embryos epithelial ethanol EtOH explants filter forceps gene expression Gibco-BRL glass human hyaluronan hybridization Incubate injection labeled limb bud membrane mesenchymal mesoderm Methods mg/mL mice microscope Molecular mouse mRNA muscle mutation NaCl needle neural tube Note oocytes overnight paraformaldehyde Pax3 pellet Petri dish phenotype plasmid prepared probe procedure prostate protein reagents recombination remove Rinse RNase room temperature RT-PCR sample secondary antibody sections segmental plate serum Sigma slides somite specific specimen sperm stage staining sterile stock solution stored at 20°C Subheading substrate supernatant tion transcription transfer transgene trophoblast trophoblast cells trypsin tumor vitro volume Wash