Physical-chemical and Immunochemical Properties of Human Ceruloplasmin and Some of Its DerivativesUniversity of Wisconsin--Madison, 1962 - 496 |
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0.1 ionic strength absorbancy ratio absorption acetate buffer agar gel amino acid anti-human ceruloplasmin antibody antigen antiserum apoceruloplasmin APOCERULOPLASMIN DEDTC apoprotein approximately ascorbate reduction product ascorbate treated ceruloplasmin ascorbic acid atoms of copper barbital blue color blue component borate buffer containing ceruloplasmin level colorless component copper atoms copper per molecule copper protein crystalline ceruloplasmin cyanide cysteine DEAE-cellulose DEDTC apoceruloplasmin denaturation determined dialysis dialyzed diffusion elution employed FIGURE gradient groups human ceruloplasmin immunoelectrophoresis increase ionic strength ionization loss of blue mercaptoethanol method minutes Lower frame mobility molecular weight molecule of protein NaCl native and apoceruloplasmin NATIVE CERULO native ceruloplasmin native protein obtained oxidase activity patterns phoresis plasmin potassium phosphate potassium phosphate buffer precipitate precipitin precipitin band presented in Fig protein solution reacting copper reduced reduced-alkylated sample sera spectra Spectrophotometric spectrum starch gel studies subunits titration Tris-borate-versene tyrosine tyrosyl residues ultracentrifuge urea versene Wilson's Disease