Calpain Methods and Protocols

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Springer Science & Business Media, Jan 1, 2000 - Science - 343 pages
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In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of u- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains."
 

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Contents

sis of Cortactin by Calpain in Platelets and In Vitro
3
r Analysis of p94 and Its Application to Diagnosis
75
Brain
101
ymograph Assessment of i and mCalpain Activity After
117
mmunologic Technique for Assessing Calpain Activation
151
fic Tissues
171
mt Assessment of Calpain and Caspase3 Activity
218
An In Vitro Assay
232
n Vivo Assay of Calpain Activity Using a Fluorescent
244
Methods in Hepatic IschemiaReperfusion Injury
260
ial IschemiaReperfusion Injury and Proteolysis of Fodrin
267
in the Lens and Cataractogenesis
276
B Shea
319
if Apoptosis
337
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