Calpain Methods and Protocols
John S. Elce
Springer Science & Business Media, 2000 - Science - 343 pages
In Calpain Methods and Protocols, John S. Elce and a seasoned team of principal investigators present a set of proven and easily followed protocols for studying calpain. The methods include in vitro techniques for the detection, expression, purification, and assay of u- and m-calpain, supplemented with a wide range of system and tissue models for studying both the physiological functions and the effects of inhibitors on calpain. The systems used include neural tissue, kidney, liver, the eye, and membrane fusion in muscle and erythrocytes, each in connection with hypoxia or other injury. Among the analytical techniques employed are casein zymography, immunofluorescence, and calpain activity assays. The authors also examine specific substrates that have been proposed for the calpains. Highly practical and readily repeatable, Calpain Methods and Protocols offers investigators involved in basic and clinically oriented calpain research a gold-standard collection of powerful experimental tools for discovering the nature and function of calpains."
What people are saying - Write a review
We haven't found any reviews in the usual places.
sis of Cortactin by Calpain in Platelets and In Vitro
r Analysis of p94 and Its Application to Diagnosis
ymograph Assessment of i and mCalpain Activity After
mmunologic Technique for Assessing Calpain Activation
mt Assessment of Calpain and Caspase3 Activity
An In Vitro Assay
Other editions - View all
20 mM Tris-HCl acid aliquots antibody apoptosis assay autolysis binding Biochem Biol blotting brain Ca2+ Ca2+-dependent CaCl2 calcium calcium-dependent calpain activity calpain inhibitor Calpain Methods calpain-mediated calpastatin casein cells centrifugation Chem chromatography coli column containing cortactin culture cytosolic degradation detected diluted domain EDTA EGTA eluted enzyme erythrocyte extract final concentration fluorescence fodrin FPLC fractions fusion gel sample buffer gradient hepatocytes homogenization human Humana Press Inc immunoblotting incubation inhibition injury integrin intracellular J. S. Elce lysate lysis buffer m-calpain medium membrane Methods and Protocols Methods in Molecular mg/mL mMEDTA mMNaCl Molecular Biology muscle mutant myoblast NaCl Note pellet peptide phenyl-Sepharose platelets PMSF protease protein proteinase proteolysis proteolytic Protocols Edited proximal tubules purification reagent receptors resin room temperature SDS-PAGE serum sodium solution specific spectrin stored Subheading substrate subunit sulfate supernatant suspension tissue Totowa tube vitro wash buffer Western blotting