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17-ketosteroids 17-OH-corticoids 17-OH-corticosteroids 17a-Estradiol Acetate acetylated acid hydrolysis ACTH adrenal aliquot amniotic fluid analysis by GLC analyzed androgens androsterone application of GLC argon biological fluids biosynthesis blood C19 steroids cancer chromatograms clinical screening column compounds concentration corticoids cortisol crude extracts decrease detectable amounts detector cell DHEA diseases eluate enzymatic hydrolysis enzyme enzyme systems Estradiol estriol estrogens Estrone Etio etiocholanolone excretion Flash heater florosil fluids and tissues fractionation gas chromatograms gas liquid chromatography GLC analysis glucosiduronates glucuronic acid hydrocele hydrocele fluid hydrolytic procedures hydrolyzed indicated ketosteroids Klendshoj metabolites method migration normal obtained ovarian fluid ovary paper chromatography patients percent SE-52 plasma polar precursors pregnanediol preparation present progesterone purification quantitation reference standard relative retention separated solvent solvolysis specimens spinal fluid steroid analysis steroid conjugate residue steroid peak sulfate T-xs Table temperature Testosterone thin layer tion unknown peak urinary urine utilizing