Animal Cell Culture TechniquesMartin Clynes Cell culture techniques allow a variety of molecular and cell biological questions to be addressed, offering physiological conditions whilst avoiding the use of laboratory animals. In addition to basic techniques, a wide range of specialised practical protocols covering the following areas are included: cell proliferation and death, in-vitro models for cell differentiation, in-vitro models for toxicology and pharmacology, industrial application of animal cell culture, genetic manipulation and analysis of human and animal cells in culture. |
Contents
| 3 | |
Screening for Mycoplasma Contamination | 38 |
SerumFree Media | 54 |
Screening Culture Media | 67 |
Generation of Monoclonal Antibodies | 82 |
The Generation of LongTerm Human Antibody | 105 |
Baculoviral Expression and Partial Purification | 131 |
Methods for Studying Phosphoinositide Metabolism | 149 |
Isolation Cultivation and Differentiation | 357 |
Culture Systems for Hepatocytes for Use in Toxicology | 371 |
Cellular Models for In Vitro Toxicity Testing 395 | 394 |
Miniaturized In Vitro Methods in Toxicity Testing | 423 |
Investigation of Antitumor Drug Accumulation | 434 |
Current Status of Genetically Engineered | 449 |
ScaleUp of Animal Cell Cultures | 459 |
Purification of Monoclonal Antibodies Produced | 475 |
Analysis of Cell Cycle and Cell Death Mechanisms | 170 |
Assessing Release of Secretory Products | 197 |
The GranulocyteMacrophage ColonyForming | 217 |
Investigations into Cell Differentiation Using Cells | 231 |
Cell Culture Models for Mononuclear Phagocytes | 240 |
The Human Leukemia Cell Line HL60 as a Cell Culture | 264 |
Cell Culture Models | 298 |
Ion Channels and Cell Signaling in Cell Cultures | 319 |
Cytokine Cell Culture Assays | 521 |
Antisense Methods in Cell Culture | 545 |
Methods for Studying the Transcription and DNase I | 567 |
Analysis of Specific and Differentially Expressed | 582 |
Review and Outlook | 600 |
| 613 | |
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Common terms and phrases
acid activity agar antigen apoptosis assay baculoviral baculovirus buffer Ca2+ Cancer cell culture cell lines cell suspension cell type cells/ml cellular centrifugation cloning cm² colonies concentration containing coverslips culture medium cyclin cytokines detection differentiation dilution DMSO drug EDTA elution endotoxin enzyme epithelial cells ethanol expression fetal filter flask fluorescence gene growth factor growth medium H₂O hepatocytes HL-60 cells human Incubate infection inoculated inositol inositol phosphates insect cells intracellular isolated lung lysate macrophages membrane method mg/ml microscope monoclonal antibodies monolayer murine mycoplasma NaCl neutrophils pellet petri dishes phosphate pipette plaque plates prepared Procedure protein protocol recombinant remove resuspend ribozyme room temperature sample sensitive serum serum-free Sigma sodium solution specific staining sterile stored supernatant techniques tion tissue culture toxicity transfection trypsin tube tumor type II cells vector viral virus viruses vitro volume wash µg/ml