PCR Technology: Principles and Applications for DNA AmplificationThis is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years. |
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Page 60
... Higuchi , R. , Horn , G.T. , Mullis , K.B. , and Erlich , H.A. ( 1988 ) Science 239 : 487 . 5. Stoflet , E.S. , Koeberl , D.D. , Sarkar , G. , and Sommer , S.S. ( 1988 ) Science 239 : 491 . 6. Wrischnik , L.A. , Higuchi , R.G. ...
... Higuchi , R. , Horn , G.T. , Mullis , K.B. , and Erlich , H.A. ( 1988 ) Science 239 : 487 . 5. Stoflet , E.S. , Koeberl , D.D. , Sarkar , G. , and Sommer , S.S. ( 1988 ) Science 239 : 491 . 6. Wrischnik , L.A. , Higuchi , R.G. ...
Page 168
... Higuchi , R. , Erlich , H.A. , and Kazazian , H.H. , Jr. ( 1987 ) Nature 330 : 384 . 18. Engelke , D.R. , Honer , P.A. , and Collins , F.S. ( 1988 ) Proc . Natl . Acad . Sci . USA 85 : 544 . 19. Saiki , R.K. , Gelfand , D.H. , Stoffel ...
... Higuchi , R. , Erlich , H.A. , and Kazazian , H.H. , Jr. ( 1987 ) Nature 330 : 384 . 18. Engelke , D.R. , Honer , P.A. , and Collins , F.S. ( 1988 ) Proc . Natl . Acad . Sci . USA 85 : 544 . 19. Saiki , R.K. , Gelfand , D.H. , Stoffel ...
Page 207
... Higuchi , Sean Walsh , and Robert Griffith for their contributions in developing and applying the HLA typing system described here . We are also grateful to Chu - An Chang for the synthesis of the HRP - labeled oligonucleotides ...
... Higuchi , Sean Walsh , and Robert Griffith for their contributions in developing and applying the HLA typing system described here . We are also grateful to Chu - An Chang for the synthesis of the HRP - labeled oligonucleotides ...
Contents
PART ONE BASIC METHODOLOGY | 1 |
PCR Amplification of Specific Sequences from | 9 |
Taq DNA Polymerase | 17 |
Copyright | |
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PCR Technology: Principles and Applications for DNA Amplification Henry Erlich No preview available - 1989 |
Common terms and phrases
Acad Acids Res agarose alleles amplification amplification products amplified DNA analysis annealing assay automated B-globin cDNA cells Cetus chromosome cloned concentration containing cycles deletion denaturant denaturing gradient gel detection DGGE diagnosis direct sequencing disease DNA fragments DNA sequences dNTP dot-blot dystrophin Erlich exon Faloona Figure flanking GC-clamp Gelfand gene genetic genomic genomic DNA heteroduplexes Higuchi HPRT human hybridization incubation individual inverse PCR lane Lerman loci locus markers melting behavior melting domain method misincorporation mismatch molecular molecules mRNA Mullis mutations Natl Nucl nucleotide oligonucleotide primers PCR amplification PCR buffer PCR fragments PCR primers PCR product PCR reaction pmol polymerase chain reaction polymorphism primers Proc procedure protein Proteinase Proteinase K Protocol recombination region restriction enzyme reverse transcriptase Saiki Scharf sequencing reactions single-stranded specific ssDNA strands synthesis Taq DNA polymerase Taq polymerase target sequence temperature template Tris.HCl tube