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1919 experiment absence of glucose ammonia ammonium nitrate ammonium sulphate amount of fixation amount of growth analyses of mineral asparagine Average all cul Average all cultures Average or Gain Azotobacter forms bacteria calcium nitrate Check Ster Chlorella vulgaris Beyr Chlorophyceae combined nitrogen cotton plugs culture flask Culture Growth Total culture solution Culture Total Mg cultures of green delivery tube distilled water Erlenmeyer flask fixation occurred Found Medium four species free nitrogen glucose Culture Total glucose or mannite Grams Medium 100 green algae green plants Growth period growth produced inoculated legume liter)—no glucose media containing Medium 100 Gr Medium without glucose milligram mineral nutrient agar mineral nutrient solution nitrate media nitrate with glucose nitrogen fixed nitrogen source nutrient agar cultures percent glucose percent mannite presence of glucose Protococcus sp Protosiphon botryoides pure culture Results of analyses Scenedesmus source of nitrogen Species N Found Species number Stichococcus sp sulphuric acid TABLES urea weight