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1-N hydrochloric acid 2k hours 30 minutes 90 cc Formaldehyde absolute alcohol Acetic acid glacial acetone acid hematein test ammonium anhydrous Barbital buffer solution buffered formalin cc Acetic acid cc Distilled water cc of dye cc Tissues celloidin changes of distilled clear and mount Counterstain decolorized Dehydrate dehydrated and embedded deparaffinized sections diluted dissolved distilled water Distilled water 100 dye solution enzyme filter fixation fixative is recommended fixed in Zenker's Formaldehyde formalin formalin-calcium chloride fuchsin g Distilled g Sodium glycerine glycerine jelly glycerol or glycerine gold chloride hematoxylin hydrochloric acid hypo Incubate lipid method methylene blue micra modified overnight oxalic acid paraffin phosphatase picric acid potassium metabisulfite prepared protargol rater reagent refrigerator Rinse in distilled room temperature running water Saturated sections from distilled silver nitrate Sodium sulfite staining in solutions stock solution substrate Sudan sulfate Tissues are fixed toluidine blue VJash Wash under tap water 100 cc xylol Zenker's fluid