Molecular Cloning: A Laboratory Manual, Volume 1 |
From inside the book
Results 1-3 of 76
Page 1-7
... restriction sites that could be used for cloning . The first plasmid to combine all of the then - available desirable features was pBR313 ( Bolivar et al . 1977a , b ) : It replicated in a relaxed fashion , contained two selectable ...
... restriction sites that could be used for cloning . The first plasmid to combine all of the then - available desirable features was pBR313 ( Bolivar et al . 1977a , b ) : It replicated in a relaxed fashion , contained two selectable ...
Page 1-59
... Restriction Sites in the Plasmid Vector and the Foreign DNA The variety of restriction sites in plasmid vectors is now extremely large , and it is often possible to find a vector that carries exactly the same restriction sites as the ...
... Restriction Sites in the Plasmid Vector and the Foreign DNA The variety of restriction sites in plasmid vectors is now extremely large , and it is often possible to find a vector that carries exactly the same restriction sites as the ...
Page 2-92
... Restriction Enzymes Vectors such as the EMBL series , 2001 , ADASH , and Charon 34 , 35 , and 40 contain a series of restriction sites , arranged in opposite orientations , in polycloning sites at each end of the central stuffer ...
... Restriction Enzymes Vectors such as the EMBL series , 2001 , ADASH , and Charon 34 , 35 , and 40 contain a series of restriction sites , arranged in opposite orientations , in polycloning sites at each end of the central stuffer ...
Contents
Essential Features of Plasmids 1 | 1-3 |
Staininging RNA Before and After Transfer to Nitrocellulose Filters 7 51 | 1-7 |
Index | 1-8 |
Copyright | |
95 other sections not shown
Other editions - View all
Common terms and phrases
activity agar agarose aliquots allow amount appropriate arms bacterial bacteriophage BamHI buffer carrying cells centrifugation Chapter cloning coli colonies competent concentration construction containing cosmid culture described digestion EcoRI efficiency electrophoresis et al ethanol ethidium ethidium bromide extracts Figure filters final foreign DNA fragments gene genetic genome gradients growth Hindill host hybridization Incubate infected inserted libraries ligase ligation linear method microfuge minutes minutes at 4°C mixture molecules mutations Nucleic Acids obtained original packaging pellet plaques plasmid DNA plate polycloning polymerase possible precipitate prepared presence probes procedure protein purified Pvul reaction recA recombinant Recover region remove replication restriction enzyme resulting room temperature Sall screening segment selection sequences single single-stranded Smal solution step sterile stored strains strand supernatant termini transfer transformation tube usually vector volume yield