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2,4-dinitrophenylhydrazine absorption acetaldehyde Acetaldehyde by Particle-Free acetic acid acetic acid fermentation Acetobacter Acetobacter peroxydans added at zero aerobic alcohol dehydrogenase aldehyde dehydrogenase aldehyde oxidation alkaline pyrogallol anaerobic conditions Atkinson Bacterial Extracts Bonnichsen buffer at pH buffer were added catalase Cell-Free Systems cent centrifugation citrate cofactor concentration crude cell-free extracts crude cell-free preparation Crude Cell-Free Systems culture cuvettes Cuvettes containing cyanide cytochromes dehydrogenase activity demonstrated dismutation DPN-linked DPNH DPNH oxidation enzyme enzyme system ethanol ethanol and acetaldehyde ethanol oxidation filled with nitrogen flasks HgOg hydrogen peroxide involved liver M/15 phosphate buffer metabolic micromoles micromoles of acetaldehyde mutase optical density oxidase oxidation of DPNH oxidations of ethanol oxidized by oxygen oxygen particle-free extract particles peroxidase peroxidase activity protein per ml Pyridine Nucleotides pyrogallol rates reaction reagents reduced TPN Reduction of Cytochromes reduction of TPN+ salicylaldehyde sidearms Spectrophotometric strates suspensions Tanenbaum Theorell tion TPNH transhydrogenase tubes whole cells