Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular BiologyFrederick M. Ausubel This volume contains shortened versions of the methods published in the looseleaf manual Current Protocols in Molecular Biology. It presents fully-tested, current techniques based on material from the core manual and from the quarterly update service. Includes all step-by-step descriptions of methods covered in the first ten chapters of CPMB and provides enough detail to perform the experiments (only introductions, annotations, and commentary have been omitted). Marginal notes explain the hows and whys of many steps, and provide tips on safety, storage, and anticipated results. Includes references and recipes for all reagents and media and helpful tables and illustrations. |
Contents
Escherichia coli Plasmids | 1 |
VECTORS DERIVED FROM PLASMIDS | 15 |
VECTORS DERIVED FROM LAMBDA AND RELATED | 28 |
Copyright | |
35 other sections not shown
Common terms and phrases
acrylamide Additional materials agarose gel aliquots ALTERNATE PROTOCOL ammonium APPENDIX aqueous BASIC PROTOCOL Bromphenol Blue buffer UNIT cDNA cDNA libraries cells Centrifuge chromatography clones column concentration containing denaturing DEPC dideoxy digestion Dilute DNA fragments DNA ligase DNA polymerase DNA sequencing DNase dNTP double-stranded EcoRI EDTA elution enzyme ethanol precipitate filter fractions gene genomic DNA glycerol gradient H₂O insert Key Reference Klenow fragment labeled ligation loading buffer mg/ml MgCl2 microcentrifuge tube mutations NaCl nitrocellulose Nucleic Acids nucleotides oligonucleotide overnight pellet phage phenol phenol extraction pipet plasmid plates Prepare primer probe protein purification radioactive reagents Remove restriction endonuclease Resuspend Rinse room temperature rotor S1 nuclease sample silanized single-stranded sodium acetate solution UNIT step supernatant SUPPORT PROTOCOL synthesis T4 DNA ligase T7 DNA polymerase Table template transfection Transfer Tris-Cl vector vortex wash µg/ml µl TE buffer