Gene Cloning : An IntroductionAn introductory textbook updated to incorporate advances made since the first edition was published in 1986, but retaining its mission to serve undergraduates with no previous experience of the subject and experienced researchers new to gene cloning. Annotation copyrighted by Book News, Inc., Portland, OR |
From inside the book
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Page 27
... cell DNA will often be required as a source of material from which to obtain genes to be cloned . Total cell DNA may be DNA from a culture of bacteria , from a plant , from animal cells , or from any other type of organism that is being ...
... cell DNA will often be required as a source of material from which to obtain genes to be cloned . Total cell DNA may be DNA from a culture of bacteria , from a plant , from animal cells , or from any other type of organism that is being ...
Page 29
... bacterial cells can be divided into physical methods , in which the cells are disrupted by mechanical forces , and ( a ) Measurement of optical density Incident light at Preparation of total cell DNA / 29 Preparation of a cell extract.
... bacterial cells can be divided into physical methods , in which the cells are disrupted by mechanical forces , and ( a ) Measurement of optical density Incident light at Preparation of total cell DNA / 29 Preparation of a cell extract.
Page 30
... Bacterial culture contained in a 1 cm thick cuvette ( b ) Estimation of cell number from a calibration curve 2.5 2.0 Optical 1.5 density at 600 nm 1.0 0.5 1 0 0.4 0.8 1.2 1.6 2.0 Cell number ( x 109 ) per ml Detector Figure 3.2 ...
... Bacterial culture contained in a 1 cm thick cuvette ( b ) Estimation of cell number from a calibration curve 2.5 2.0 Optical 1.5 density at 600 nm 1.0 0.5 1 0 0.4 0.8 1.2 1.6 2.0 Cell number ( x 109 ) per ml Detector Figure 3.2 ...
Contents
Why gene cloning is important | 3 |
plasmids and bacteriophages | 12 |
Purification of DNA from living cells | 27 |
Copyright | |
12 other sections not shown
Common terms and phrases
amino acid antibody autoradiograph bacteriophage bacterium BamHI base pairing biotechnology carried cDNA chromosomal DNA cloned gene cloning vectors coded codons coli colonies complementary containing control sequence cosmid culture cystic fibrosis cystic fibrosis gene defective deletion digestion DNA fragment DNA sequencing double-stranded EcoRI enzyme expression vector foreign gene gel electrophoresis gene cloning gene expression gene Figure genomic library higher organisms host cell hybridization probing identified infection insulin introns labelled lacZ LEU2 ligated M13 vector medium membrane method molecular molecule Figure mRNA mutagenesis mutation normal nuclease nucleotide nucleotide sequence obtained oligonucleotide phage particles plant cells plaques plasmid polylinker polymerase polynucleotide produced promoter pUC8 purified recombinant DNA recombinant DNA molecule recombinant protein region replication restriction endonuclease restriction fragment restriction sites result RFLP selectable marker signals single-stranded DNA specific sticky ends T-DNA techniques Ti plasmid transcription transformed translation product trpA types unique restriction vaccine virus viruses vitro vitro mutagenesis yeast