Synthesis of Electron Dense Labels and Structural Analysis of Multiprotein Aggregates |
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Page 76
... PMSF and 1 mM EDTA to prevent or minimize proteolysis . PMSF was added as dissolved in isopropyl alcohol . saturation in aqueous buffer , any undissolved PMSF was removed by suction filtration . The PMSF and EDTA were added to the ...
... PMSF and 1 mM EDTA to prevent or minimize proteolysis . PMSF was added as dissolved in isopropyl alcohol . saturation in aqueous buffer , any undissolved PMSF was removed by suction filtration . The PMSF and EDTA were added to the ...
Page 208
... PMSF and 0.1 mM EDTA . The subcomplexes of E1E2 and E2E3 and the component enzymes were purified from the PDH complex on the CaPO4 gel- cellulose columns according to Reed and Willms ( 1966 ) in the continuous presence of the above ...
... PMSF and 0.1 mM EDTA . The subcomplexes of E1E2 and E2E3 and the component enzymes were purified from the PDH complex on the CaPO4 gel- cellulose columns according to Reed and Willms ( 1966 ) in the continuous presence of the above ...
Page 209
Heechung Yang. pH 7.0 with 1 mM PMSF and 0.10 mM EDTA . The reconstitution mixtures were left on ice for 60 min before being cross - linked with glutaraldehyde for STEM as described E2 below . Similarly , E2 + 4E1 ( E2 core plus 2 ...
Heechung Yang. pH 7.0 with 1 mM PMSF and 0.10 mM EDTA . The reconstitution mixtures were left on ice for 60 min before being cross - linked with glutaraldehyde for STEM as described E2 below . Similarly , E2 + 4E1 ( E2 core plus 2 ...
Contents
Complexes by Electron Microscopy | 15 |
Materials and Methods | 66 |
66 | 75 |
13 other sections not shown
Common terms and phrases
acetic anhydride acylation alkylating amino groups avidin avidin molecules binding sites Bio-Gel P-6 Bleile bovine heart buffer CaJacob Chapter chemical chromatography citraconic anhydride cm column Coli PDH complex column of Sephadex component enzymes cross-linked derivatives desalted described dihydrolipoyl transacetylase dimeric E₁ E1 and E3 E₁ or E3 E₂ E2 core E2 enzymes E2E3 subcomplex E3 subunits EDTA electron dose electron-dense labels equilibrated and eluted fractions Frey gel electrophoresis glutaraldehyde gold clusters gold complex henicosa Histogram icosa N-acetyl)-1 images ligands lipoyl mass of PDH mixture molecular weight mono monomeric particle mass measurements PMSF point group prepared presence primary amino groups procedure protein purified Pyruvate Dehydrogenase Complex radial mass analysis radioactivity ratios reaction Reardon Reed rotary evaporation sample scanning transmission electron scattered electrons SDS-PAGE SDS-polyacrylamide gel electrophoresis shell masses solution SP-Sephadex C-25 species specimen STEM structural subunit binding domain symmetry synthesis transmission electron microscopy undecagold clusters