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acetic anhydride acetyl-CoA acetylation acylation alkylating amino groups avidin avidin molecules band binding sites Bio-Gel P-6 biological Bleile et al.,l979 buffer C]cysteine C]phthalic anhydride Chapter Chemical chromatography citraconic anhydride cm column Coli PDH complex column of Sephadex column of SP-Sephadex component enzymes compound concentrated by rotary cross-linked cube derivatives described dihydrolipoyl dihydrolipoyl transacetylase dimeric E2 core E2 enzymes EDTA elastically scattered electrons electron dose electron-dense labels equilibrated and eluted ferritin Figure l4 fractions Frey gel electrophoresis glutaraldehyde gold atoms gold clusters gold complex icosa(N-acetyl)-l images l0"3 X Mass ligands mass of PDH mixture molecular weight monomeric NaBH nondeca(N-acetyl)-l particle mass measurements PMSF point group presence primary amino groups procedure protein purified radial mass analysis radioactivity reaction reagents Reed rotary evaporation sample scanning transmission electron SDS-PAGE Sephadex shell masses solution SP-Sephadex C-25 species specimen STEM structures subcomplex subunit binding domain symmetry synthesis transmission electron microscopy umol undecagold clusters