Lymphocytes: A Practical ApproachG. G. B. Klaus Technology and methods in cellular immunology are rapidly improving and, at the same time, becoming increasingly diverse. This book answers the need for a basic methods handbook devoted to lymphocytes. |
Contents
Cells from Body Fluids | 9 |
Amlot | 11 |
Simple Purifications | 23 |
Copyright | |
17 other sections not shown
Common terms and phrases
activity aliquots anti-Ig anti-mouse Ig antibody response antigen antigen-specific avidin beads binding biotin blood bone marrow buffer cell lines cell suspension centrifugation clones coated complement complete medium concentration containing cytoplasmic cytotoxic dendritic cells depletion described dilution Dynabeads M-450 erythrocytes Fc receptors Figure filter FITC fluorescence fluorochromes haptens human immune Immunol Immunology Incubate labelled layer leukaemia lymph node lymphocytes lymphoid lymphokines lysis MAbs macrophages marker membrane method mg/ml microplate ml of PBS monoclonal antibody monocytes mononuclear cells mouse normal particles Pasteur pipette pellet phenotype pipette plaque assay plates polyclonal positive selection Prepare procedure protein rabbit reagents receptors Resuspend room temperature rosetting RPMI saline samples Section serum slides sodium sodium azide solution spleen spleen cells SRBC staining sterile stimulation subsets supernatant surface Table target cells Tc cells technique thymocytes tissue culture tube virus vitro Wash the cells µg/ml
References to this book
Immunochemistry: A Practical Approach, Volume 1 Alan Johnstone,M. W. Turner No preview available - 1997 |