Lymphocytes: a practical approach
G. G. B. Klaus
IRL Press, 1987 - Medical - 261 pages
Technology and methods in cellular immunology are rapidly improving and, at the same time, becoming increasingly diverse. This book answers the need for a basic methods handbook devoted to lymphocytes.
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Cells from Body Fluids
26 other sections not shown
3H]thymidine activity aliquots anti-Ig anti-mouse Ig antibody response antigen antigen-specific avidin beads binding biotin bone marrow buffer cell lines cell suspension centrifugation clones coated complement complete medium concentration containing cytoplasmic dendritic cells depletion described dilution Dynabeads M-450 erythrocytes factor Figure filter FITC fluorescence fluorochromes haptens Herzenberg.L.A. human Immunol Immunology Incubate interleukin labelled layer leukaemia lymph node lymphocytes lymphoid lymphokines lysis MAbs macrophages marker membrane method mg/ml microplate mitogen monoclonal antibody monocytes mononuclear cells mouse negative normal particles Pasteur pipette pellet phenotype pipette plaque assay plates polyclonal positive selection Prepare procedure protein rabbit reagents receptors Resuspend room temperature rosetting RPMI saline samples Section serum slides sodium sodium azide solution specific spleen spleen cells SRBC staining sterile stimulation subsets supernatant surface Table target cells technique tg/ml Th cells thymocytes tissue culture tube virus vitro Wash the cells