Regulatory Mechanisms of the Cool Family Proteins and Their Impact on Growth ControlCornell University, 2006 - 378 pages |
Contents
Physiological Mechanisms Controlling the GEF activity | 29 |
The Cool2aPix protein mediates a Cdc42Rac signaling | 68 |
The Cool1BPix protein serves as an essential regulatory | 107 |
Copyright | |
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Common terms and phrases
3T3 cells expressing ability actin activate Rac activated Cdc42 activated forms activation of Cdc42 activity of Cool-2 anti-HA antibody assay beads Biol Chem Cbl-b Cdc42 and Rac Cdc42 or Rac Cdc42-GEF activity Cell Biol cell growth cell lysates cell surface cells expressing v-Src cellular Cerione Cool proteins Cool-2 constructs Cool-2 dimer Cool/Pix proteins COS-7 cells cytoskeleton DH domain dimer dissociation EGFR endocytosis Figure focal complex forms of Cdc42 function GEF activity gene GMP-PCP GMP-PCP-bound Cdc42 GTP-binding proteins GTP-bound guanine guanine nucleotide exchange HA-Cbl-b HA-tagged His-tagged immunoprecipitated incubated inhibited integrins Invitrogen lane mediated mutant NIH 3T3 cells nucleotide exchange factor panel shows pathway phosphorylated Cool-1 phosphorylation of Cool-1 phosphorylation-defective plasmid purified Rac1 recombinant regulatory Rho GTPases Rho proteins role RSID serum SH3 domain shown shRNA signaling stimulate T1 region Tiam1 top panel transfected transformation tumor tyrosine phosphorylation ubiquitination Western blotting wild-type Cool-1