1HNMR Studies of Membrane-bound Molecules in Perdeuterated Model Membranes: Gramicidin, Valinomycin, and Specific Phospholipids |
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acid Acta activity added addition amount appears assay assume bilayer binding Biochem Biophys broadening cations cause changes Chem chemical shifts chloride choline chromatography column complex concentration conformation constant correlation dependence determined DMPC EDTA effect electron et al expected experiments fatty acyl Figure flask followed fractions freeze-thaw frequency function fusion gel phase formation glycerol gramicidin important indicated intensity involved ions KSCN lanthanide lateral lipid magnetic material measured membrane method methyl mixture molecule motion observed obtained occurs orientation peak performed phase separation phase transition phosphate phosphatidylcholine phospholipid polyvalent cations possible potassium presence probably procedure protons pyridine ratio reaction relative relaxation resonances sample shown signal signal intensity similar solution solvents sonicated spin label studies surface synthesis Table technique temperature Temperature dependence titration tube valinomycin various vesicles volume yield