Gene cloning: the mechanics of DNA manipulation
The principles of cloning DNA; Recombination and mutagenesis of DNA in vitro; Bacteriophage vectors; Bacterial plasmid vectors; Expression of cloned DNAs in E. coli plasmids; The physical characterization of cloned DNA segments and their counterparts within chromosomes; Gene cloning in fungi and plants; Expression of cloned genes in animal cells.
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Recombination and mutagenesis of DNA in vitro
Bacterial plasmid vectors
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acid amino-acid annealed antigen bacterial bacteriophage base pair carrying cDNA cDNA cloning cell lines chromosomal chromosomal DNA cleavage sites cleaved cloned DNA cloning vectors codon ColEl colicin constructed containing deletion detected developed digestion DNA ligase DNA molecules DNA polymerase DNA segments DNA sequences Drosophila EcoRI EcoRl efficient encoded enzyme Escherichia coli eukaryotic eukaryotic genes foreign DNA functional galactosidase gene product genetic genome globin gene Hindlll homologous host human hybrid infection insertion intervening sequences introduced isolated lactamase lacZ gene ligated lysogens mammalian mapping marker molecular mouse cells mRNA mutagenesis mutations Natn Acad nuclease nucleotide octopine oligonucleotide operon peptide phage plaques plasmid DNA plasmid vectors probe Proc prokaryotic promoter protein Pstl reading frame recombinant DNA region replication resistance restriction endonuclease restriction fragment screening selected specific splicing strain strand synthesis T-DNA techniques terminal transcription transformation transposon tumour viral virus vitro wild type yeast