Plant Tissue and Cell CultureLaboratory organization. Tissue (Callus) cultures - techniques. Cell (Suspension) cultures - techniques. Isolated plant protoplasts. General cytology of cultured cells. Nuclear cytology. Single-cell clones - derivation and selection. Anther and pollen culture. Growth patterns in tissue (Callus) cultures. Growth patterns in cell cultures. Aspects of organization - organogenesis, embryogenesis, cytodifferentiation. Plant tumour cells. Applications in plant pathology. Old problems and new perspectives. |
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Contents
Laboratory Organization | 11 |
Tissue Callus CulturesTechniques | 31 |
Cell Suspension CulturesTechniques | 61 |
Copyright | |
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Acer acid activity aggregates anther appear auxin callus carrot cell cultures cell division cell suspension changes Chapter chromosomes concentration containing continuous crown-gall cycle cytoplasm density derived differentiation dividing effect embryos enzymes established experiments explants factors formation further fusion gametophytic genetic glucose growing growth growth rate haploid host hybrid important increase incubation induced infection initial involved isolated light limited lines material medium method mycelium Nicotiana normal nuclei nutrient observed obtained occur organ origin particular period phase plant cells plant tissue plating pollen population possible preparation present production protein protoplasts regeneration reported resistance root selection separation showed shown similar single species stage sterile strain Street structure studies suggested surface suspension cultures synthesis tabacum techniques tion tissue cultures tobacco transfer treatment tumour virus vitro volume wall weight