A Textbook of bacteriology

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D. Appleton & Company, 1922 - Medical bacteriology - 1193 pages
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Page 787 - Carefully sterilize the syringe after injecting each horse by naming the needle over an alcohol lamp or, better, use separate syringes for healthy and suspected animals. If the same syringe is used, inject the healthy animals first, and flame the needle of the syringe after each injection. "Take the temperature every two hours for at least eighteen hours after the injection.
Page 1017 - Procedure. — Shake at least twenty-five times the bottle which contains the sample. Withdraw 1 ce of the sample with a sterilized pipette and deliver it into a sterilized Petri dish, 10 cm. in diameter. If there be reason to suspect that the number of bacteria is more than 200 per cc, mix 1 cc of the sample with 9 cc of sterilized tap or distilled water.
Page 762 - ... approximately one per two fields, and a culture shows an absence of hemolytic cocci, the wound is considered susceptible of secondary suture except when the wound has contained hemolytic cocci at any time. In that case careful cultures are made from granulation tissue and from the discharge from all parts of the wound, and absence of hemolytic cocci should be established by two successive negative cultures before suture is made. It has been observed that streptococci are prone to lie dormant...
Page 587 - Five hundred grams of beef or veal are infused in 500 cc of a 15 per cent, solution of glycerin in water. Twentyfour hours later the meat is squeezed in a sterile press and the infusion collected in a sterile beaker.
Page 817 - ... the floor above. The wall must fit tightly to the flooring and not merely extend to the joists or supporting timbers, as this would result in open spaces for the entrance of rodents. Groceries, stables, warehouses, markets and food depots in general are best rat-proofed by having a concrete floor in addition to the walls. In...
Page 1133 - The heating should not be done in a pressure sterilizer, nor in a water bath, nor in any other way than as stated. When cool, pour the mixture into a large vessel and add to it, stirring or shaking meanwhile, 500 ccm.
Page 774 - Although the bacilli are not demonstrable in the blood until just before death, they nevertheless invade the blood and lymph streams immediately after inoculation, and are conveyed by these to all the organs. This has been demonstrated clearly by experiments where inoculations into the tail or ear were immediately followed by amputation of the inoculated parts without prevention of the fatal general infection. The bacilli are probably not at first able to multiply in the blood. At the place of inoculation...
Page 1133 - ... while on close inspection a finely granular black precipitate appears in suspension.
Page 132 - ... keeping the section in constant motion so that the decolorization shall be uniform. Control the result under the microscope. When the pink color has returned to the section and the nuclei are still a deep blue, finish the dehydration quickly with absolute alcohol.
Page 787 - The temperature as a rule, will begin to rise from four to eight hours after the injection, and reach its maximum from ten to sixteen hours after injection. On the day succeeding the injection take the temperature at least three times. " In addition to the febrile reaction, note the size, appearance, and duration of any local swelling at the point of injection. Note the general condition and symptoms of the animal, both before, during, and after the test. "Keep the solution in the sealed bottle and...

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