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Applications of Cryogenic atoms bacteriophage biochemical biological specimens biological systems Biophysics Boersch Cell Research coils components cooling cryo cryo-electron microscopes cryofixation Cryogenic Cryogenic Technology Cryomicroscopy cryostat crystalline Dewar diamond knife electron diffraction electron diffraction patterns electron micro electron micrographs electron microscopy electron optical embedding experimental Exptl Fernandez-Moran films free radicals freeze-substitution frozen glycerol high-resolution electron microscopy hydrated ice crystals ice-crystal images improved kilogauss Laboratory lamellar systems lattice layers lipoprotein liquid helium temperatures liquid nitrogen low temperatures low-temperature preparation techniques macromolecular magnetic fields membranes microbeam illumination microtome molecular myelin sheath objective lens observed obtained operating organization persistent current mode photoreceptors Phys pole pieces possible preservation Proc radiation damage rapid freezing recorded resolution electron Schmitt sections single-crystal solenoid solenoid lenses stability structure superconducting lenses superconducting solenoid superfluid superfluid helium refrigerator thermal thermal noise thin tion tissue ultrastructure ultrathin University of Chicago vacuum X-ray diffraction