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Acad Sci USA acquired immunodeficiency syndrome AIDS patients amino acid anti-HIV antigen antiviral assay asymptomatic binding CD4+ cell lines cell number cellular clinical clone concentrations containing culture cytopathic effect cytotoxic detected dilution disease ELISA expression extracts Gallo RC genome glycoprotein H9 cells HIV antibody HIV infection homosexual HTLV-III HTLV-III/LAV HTLV-IV human immunodeficiency virus HUMAN RETROVIRUSES human T-lymphotropic virus immune incubated infected cells inhibition Interferon isolates lane lymphocytes lysate Mary Ann Liebert medium molecular monoclonal antibody Montagnier National Cancer Institute Natl Acad Sci neutralizing antibodies nucleotide patients with AIDS peptide Ph.D positive Proc Natl Acad production prostitutes purified rabbit reacted reactivity recombinant region replication response retroviruses reverse transcriptase RT activity Science sera seroconversion seronegative seropositive shown simian specific supernatant T-cell Table tested titers transfected tumor uninfected virus type viruses vitro Western blot Wong-Staal