IntegrinsAn integrin, or integrin receptor, is an integral membrane protein in the plasma membrane of cells. It plays a role in the attachment of a cell to the extracellular matrix (ECM) and to other cells, and in signal transduction from the ECM to the cell. There are many types of integrin, and many cells have multiple types on their surface. Integrins are of vital importance to all metazoans, from humans to sponges. This volume in Methods in Enzymology presents methods for studying integrins. |
Contents
| 1 | |
Investigating Integrin Regulation and Signaling Events in ThreeDimensional Systems | 27 |
Integrins in Cell Migration | 47 |
Integrin Cytoskeletal Interactions | 69 |
Cell Survival in a ThreeDimensional Matrix | 85 |
Platelet Integrin Adhesive Functions and Signaling | 103 |
Development of Monoclonal Antibodies to Integrin Receptors | 117 |
Cell Adhesion Cellular Tension and Cell Cycle Control | 155 |
Identification and Molecular Characterization of Multiple Phenotypes in Integrin Knockout Mice | 291 |
Purification Analysis and Crystal Structure of Integrins | 307 |
Electron Microscopy of Integrins | 337 |
Intravital Imaging and Cell Invasion | 375 |
Using Xenopus Embryos to Investigate Integrin Function | 403 |
Methods to Study Lymphatic Vessel Integrins | 415 |
Analysis of Integrin Signaling in Genetically Engineered Mouse Models of Mammary Tumor Progression | 439 |
Design and Chemical Synthesis of Integrin Ligands | 463 |
Analysis of Integrin Signaling by Fluorescence Resonance Energy Transfer | 177 |
Studies on Integrins in the Nervous System | 203 |
Methods for Identifying Novel Integrin Ligands | 223 |
Analysis of Integrin Functions in PeriImplantation Embryos Hematopoietic System and Skin | 239 |
Evaluating Integrin Function in Models of Angiogenesis and Vascular Permeability | 505 |
| 529 | |
| 559 | |
Other editions - View all
Integrins: Molecular and Biological Responses to the Extracellular Matrix David A. Cheresh,Robert P. Mecham No preview available - 1994 |
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acid affinity aIIbb3 alpha analysis angiogenesis angiogenic apoptosis assay avb3 avb5 beta binding biosensor buffer cancer cell adhesion Cell Biol cell culture cell lines centrifugation Chem Cheresh collagen collagen gels complex confocal containing coverslips crystal cytoplasmic cytoplasmic domain cytoskeleton density diluted Edited embryos endothelial cells expression extracellular matrix fibrinogen fibronectin filter fluorescence focal adhesion gene growth factor heterodimers Hynes imaging immune incubated injected integrin alpha integrin function integrin receptors interactions Invitrogen keratinocytes kinase knockout laminin ligand lymphatic mediated medium membrane metastasis methods mg/ml mice microscopy migration molecular molecules monoclonal antibodies mouse neurons particles peptide plate platelet podosomes Procedure protein protocol purified regulation resuspended role room temperature sample secondary antibody serum skin solution specific Src biosensor staining structure studies substrate subunit supernatant surface tail transfection tumor tumor cells vascular vitro vivo VOLUME washed window chamber
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