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Airy disc Airy pattern amplitude atoms back focal plane bushy stunt virus central maximum configuration continuous helical projection convolution theorem define demonstrate described diffraction grating diffractometer discuss electron micro electron micrographs electron microscope equation 60 example ferritin film emulsion Formvar Fourier analysis Fourier optics Fourier synthesis Fourier transform Fraunhofer diffraction granularity helical structures helix high-magnification Hilbert transform holes illumination image creation image formation image interpretation image plane image profile inserted integral inverse label layer lines mathematical molecules monomer unit noise number of strands object mask object profile objective lens optical diffraction pattern optical diffractometer optical shadowing Optical Transform phase grain plot radiation Reconstructed image relative intensities repeat distance represent scatter selection rule set of points shows single strand slope spatial filter spatial frequencies specimen substructure subunit symmetrical arrangements theoretical calculations three dimensional tion pattern transmission function virus particles wave wavefunction X-ray diffraction X-ray fiber zero zero-order diffraction spot