Handbook of Basic Microtechnique
Materials and processes of slide-making; Fixation and fixatives; Stains and Staining; Dehydrating and clearing; Mounts and Mountants; Making wholemounts; Making sections; Cleaning, labeling, and storing slides.
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Materials and Equipment
Stains and Staining
Dehydrating and Clearing
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70 per cent absolute alcohol angle arthropods block bottle Bryozoa carmine celestin blue cells cent alcohol cent solution Chromic acid clean clearing agent color comes containing coverslip cutting facet dehydrating dehydrating agent described differentiating solution diluted dioxane dish dissolved distilled water dried balsam drop edge embedding embryo employed examined finger bowl fixation fixative flattened fluid formaldehyde hardened hematoxylin holder impregnated iodine knife label layer material medium melting mercuric chloride method microscope microtome minutes miscible mixture mordant mountant mounted in balsam narcotized natural balsam necessary nuclei object overnight paper paraffin picric acid pipette placed potassium Potassium dichromate prefers preparation purpose quantity reagent recommended removed resin ribbon Rinse running water safranin sections shown in Fig slide smear solvent specimen staining solution sufficiently surface tap water technique temperature terpineol thick thin tion tissues transferred tube usually vial warm washed in running watch glass wholemounts worms xylene