Molecular Biology of Nucleases
Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.
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Damage Specific Nucleases
Sugar NonSpecific Nucleases
Molecules that Interact with Nucleases
Biological Function of Nucleases
Nucleases and Human Diseases
Nucleases as Tools
Acad amino acid amino acid sequence AP endonuclease assay bacterial bacteriophage base binding Biochem catalyzes cells characterized chromosome cleavage cleave cloned damaged defective deoxyribonuclease described digestion dimer DNA chain DNA molecules DNA polymerase DNA repair DNA replication DNA segments DNase double strand breaks Drosophila duplex EcoRI Escherichia coli eukaryotic excision repair exodeoxyribonuclease exonucleolytic function gene encoding gene product genetic genome gyrase heteroduplex Holliday structure homologous identified inhibited inhibitors integrase intron invertase Kdal mammalian methylation mismatch repair mitochondrial molecular weight mRNA mutants Natl Neurospora nick nuclease activity Nucleases involved nucleic acid nucleotide nucleotide sequence oligonucleotides pancreatic phage phosphodiester plasmid possess Proc prokaryotes proofreading properties protein purified recombinase region repair pathways resolvase restriction endonucleases ribonuclease ribozyme RNase RNase activity role of nucleases shown specific recombination splicing substrate subunit supercoiled synthesis topoisomerase topoisomerase II transcription transposases tRNA viral virus vitro wild type yeast
Page 249 - The bacteriophage lambda int gene product. A filter assay for genetic recombination, purification of Int, and specific binding to DNA.
Page 238 - DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.
Page 247 - Arber. 1982. A site-specific, conservative recombination system carried by bacteriophage PI . Mapping the recombinase gene ein and the cross-over sites cix for the inversion of the C segment.
Page 249 - Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.
Page 269 - RecBC enzyme nicking at Chi sites during DNA unwinding: location and orientation-dependence of the cutting.