Molecular Biology of Nucleases

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CRC Press, Mar 6, 1995 - Medical - 296 pages
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Nucleases occupy a central position in the biochemistry of DNA transactions and other metabolism of nucleic acids in all organisms. They have also proven useful in modern biological studies crucial for the development of recombinant DNA technology and reverse genetics. Nucleases assist in the identification and characterization of genes responsible for several diseases and their possible alleviation by gene therapy. Molecular Biology of Nucleases introduces the properties and biological roles of nucleases. It is the one comprehensive source for newcomers to the field.
 

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Contents

About the Author
1
X
3
Restriction Endonucleases
59
Damage Specific Nucleases
75
Topoisomerases
83
Recombinases
95
XI
99
Sugar NonSpecific Nucleases
121
NonProtein Nucleases
129
Molecules that Interact with Nucleases
143
Biological Function of Nucleases
149
Transcription
185
Nucleases and Human Diseases
193
Nucleases as Tools
203
Chap
204
Copyright

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Page 249 - The bacteriophage lambda int gene product. A filter assay for genetic recombination, purification of Int, and specific binding to DNA.
Page 249 - BROWN. 1976. Function of gene 49 of bacteriophage T4. II. Analysis of intracellular development and the structure of very fast-sedimenting DNA.
Page 238 - DNA topoisomerase II mutant of Saccharomyces cerevisiae: topoisomerase II is required for segregation of daughter molecules at the termination of DNA replication.
Page 247 - Arber. 1982. A site-specific, conservative recombination system carried by bacteriophage PI . Mapping the recombinase gene ein and the cross-over sites cix for the inversion of the C segment.
Page 249 - Determination of the number of superhelical turns in simian virus 40 DNA by gel electrophoresis.
Page 269 - RecBC enzyme nicking at Chi sites during DNA unwinding: location and orientation-dependence of the cutting.
Page 270 - M. (1984). Isolation of type I and II DNA topoisomerase mutants from fission yeast: single and double mutants show different phenotypes in cell growth and chromatin organization.

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