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In Vivo Analysis of lacZ Fusion Genes in Transgenic STEPHEN SMALL
The STag Fusion System for Protein Purification Ronald T RAINES
Tagging Exported Proteins Using Escherichia coli COLIN MANOIL
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acid activity added addition affinity allow amino analysis antibody applications assay bacteria bacteriophage binding Biol biotinylated blue buffer carrying cells chromatography chromosomal cleavage cloning coli colonies column concentration construct containing culture cytoplasm described detection determined dilution Edited efficient encoding enzyme example expression extract final fluorescence fragment functional fusion protein gene growth HIS3 host identify increase Incubate induced infection insertions integrated intensity isolated levels light luciferase measured medium Methods mutations N-terminal Note peptide performed phage plasmid plate positive preparation present primer procedure promoter protocol provides purification reaction recombinant removed reporter residues sample selection sensitivity sequence signal solution sorting specific step strain Strep-tag substrate temperature terminus tion transcriptional transfer tube vectors VOLUME washed Western blot