Short Protocols in Molecular Biology: A Compendium of Methods from Current Protocols in Molecular Biology
Frederick M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J. G. Seidman, John A. Smith, Kevin Struhl
John Wiley & Sons, Oct 6, 1995 - Science - 900 pages
A desktop companion to the three-volume Current Protocols in Molecular Biology, the recognized leader in bioscience laboratory manuals. This edition contains over 220 protocols from leading laboratories worldwide. All methods are lab-tested and include step-by-step instructions, equipment and materials necessary to successfully conduct an experiment.
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PREPARATION AND ANALYSIS OF
5B PulsedField Gel Electrophoresis 215
18 other sections not shown
acetate acid acrylamide Additional Materials agarose aliquots alkaline phosphatase ALTERNATE PROTOCOL ammonium ammonium persulfate amplification analysis antibody antigen assay Basic Protocol binding buffer centrifuge cloned coli colonies column concentration containing Contributor culture denaturing detection digestion dilution dish DNA fragments DNA polymerase DNA Sequencing DNase electrophoresis elution enzyme ethanol ethanol precipitate ethidium bromide extract filter gene genomic glass gradient hybridization ice-cold Incubate insert labeled Mammalian Cells Materials see appendix medium membrane mg/ml microcentrifuge tube mutation nitrocellulose oligonucleotide optimal overnight peptide pipet plasmid plate precipitate Prepare primer probe protein purified reaction recombinant Remove restriction endonuclease reverse transcriptase Rinse room temperature sample selection single-stranded slide sodium sodium acetate specific staining step sterile substrate supernatant Support Protocol Table template tissue transcription transfection transfer transformation ug/ml unit vector viral virus volume vortex wash yeast