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Basic Concepts of Protein Purification and Characterization
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activity addition affinity allow altered amino acid amount apparent approach binding bonds bound Chap chemical chromatography complex concentration conformational changes considered constant containing cross-linking defined dehydrogenase dependence derivatives described detection determined discussed dissociation effects elution energy enzyme equation equilibrium examine example exchange experiment experimental Figure fluorescence followed formation fragments function give given glutamate increased indicated inhibitor initial interactions involved kinetic labeled lead ligand ligand binding material matrix measurements mechanism methods modification molecular weight molecule observed obtained occur parameters particular peptide phase plot polypeptide position possible presence problem properties protein purification reaction reagents regions release residues result secondary separation sequence shown in Fig shows side chains solution specific step structure studies substrate subunits Table tion usually values variety various versus