Fluorescence Fluctuation Spectroscopy (FFS), Part 1

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Academic Press, Dec 28, 2012 - Science - 336 pages
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation Spectroscopy
Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.
  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers fluorescence fluctuation spectroscopy
  • Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.
 

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Contents

Contributors
ix
Preface
xi
Methods in Enzymology
xiii
40 Years of FCS How It All Began
xlix
Brief Introduction to Fluorescence Correlation Spectroscopy
11
DualColor Fluorescence CrossCorrelation Spectroscopy with Continuous Laser Excitation in a Confocal Setup
43
Brightness Analysis
71
TimeIntegrated Fluorescence Cumulant Analysis and Its Application in Living Cells
99
Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy
145
DualFocus Fluorescence Correlation Spectroscopy
175
Pulsed Interleaved Excitation Principles and Applications
205
Image Correlation Spectroscopy Mapping Correlations in Space Time and Reciprocal Space
245
Author Index
269
Subject Index
279
Color Plate
287
Copyright

Raster Image Correlation Spectroscopy and Number and Brightness Analysis
121

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