Atomic Force Microscopy For Biologists (2nd Edition)Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the 'natural' conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems.The second edition of this bestseller has been updated to describe the latest developments in this exciting field, including a brand new chapter on force spectroscopy. The dramatic developments of AFM over the past ten years from a simple imaging tool to the multi-faceted, nano-manipulating technique that it is today are conveyed in a lively and informative narrative, which provides essential reading for students and experienced researchers alike./a |
Contents
1 | |
5 | |
CHAPTER 3 BASIC PRINCIPLES | 41 |
CHAPTER 4 MACROMOLECULES | 76 |
CHAPTER 5 INTERFACIAL SYSTEMS | 181 |
CHAPTER 6 ORDERED MACROMOLECULES | 231 |
Other editions - View all
Atomic Force Microscopy for Biologists V. J. Morris,A. R. Kirby,A. P. Gunning No preview available - 1999 |
Common terms and phrases
adhesion adsorbed adsorption AFM images AFM studies AFM tip antibodies aqueous atomic force microscopy bacterial bacteriorhodopsin binding biofilms Biol biological Biophys biopolymers buffer cantilever cell wall cellulose Chem chromatin chromosomes collagen colloidal complexes contact mode crystals deflection deformation displacement DNA molecules domains DPPE dynamic effect elastic electron microscopy fibres Figure force spectroscopy force-distance curves gels granules Hansma helical high resolution interactions interface interfacial film Langmuir laser layer lipid living cells loop mapping Marszalek measurements mechanical membrane proteins method mica mode imaging molecular molecules monolayers Morris Müller Nucleic Acids observed obtained optical optical tweezers particles phospholipid bilayers Phys piezoelectric PNAS USA polymer polysaccharide possible regions resolution images revealed sample surface scanner scanning force microscopy scanning probe microscopy Scanning tunnelling microscopy single molecule solution spring constant starch Struct structure substrate surfactant Tapping mode technique thermal topography Ultramicroscopy visualise Zhang