DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition
CRC Press, Feb 28, 2005 - Science - 472 pages
Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results.
This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application.
The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.
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Chapter 2 Detecting DNA Variation by Molecular Markers
Chapter 3 Laboratory Equipment
Chapter 4 Methodology
Chapter 5 Evaluation of Molecular Marker Data
Chapter 6 Applications of DNA Fingerprinting in Plant Sciences
Chapter 7 Linkage Analysis and Genetic Maps
SNiPs and Chips for DNA and RNA Profiling
AFLP agarose gels alleles allozyme analyzed Appl Arabidopsis banding patterns Biol BioTechniques buffer cDNA cell centrifugation Chapter chloroplast chromosome clones codominant concentration cpDNA cpSSR CTAB cultivars denaturing detected digested DNA extraction DNA fingerprinting DNA fragments DNA isolation DNA markers DNA polymerase DNA profiling DNA sequence dNTPs Ecol ethidium bromide Evol gene flow genetic diversity genetic mapping genetic variation genomic DNA genotypes haplotypes homoplasy hybridization ISSR labeled large number ligated linkage method microsatellite microsatellite loci microsatellite markers microsatellite primers minisatellite molecular markers multilocus mutation nuclear Nucleic Acids Res nucleotide oligonucleotide PAA gels PCR primers PCR products phylogenetic phylogeographic pipet Plant Mol plant species polysaccharides population genetics primer pairs primers probes procedure proteins protocol random amplified RAPD RAPD fragments reaction regions restriction enzymes retrotransposons RFLP S-SAP samples selective SNPs solution staining step studies target taxa technique template DNA Theor tissue transposons
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