DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition

Front Cover
CRC Press, Feb 28, 2005 - Science - 472 pages
3 Reviews
Given the explosive development of new molecular marker techniques over the last decade, newcomers and experts alike in the field of DNA fingerprinting will find an easy-to-follow guide to the multitude of techniques available in DNA Fingerprinting in Plants: Principles, Methods, and Applications, Second Edition. Along with step-by-step annotated protocols, the authors fully discuss the technical aspects and modifications of existing techniques, the influence of reaction components and conditions, and the analysis of the results.

This second edition has been completely revised to address the exponential changes in the field since the first edition, focusing on PCR-based techniques but also including more sophisticated ones. The authors include numerous case studies to illustrate applications of the methods, more than 1600 references to the literature, and descriptions of reagent formulation, equipment, and computer programs used for evaluating molecular marker data. They compare the various methods, including the costs and benefits of each, helping readers determine which is best suited to a particular application.

The well-rounded, cross-referenced, and unified nature of this book makes it intrinsically easier to follow than the edited, multi-authored books currently available. It is an absolute necessity on the lab bench of anyone involved in plant research, DNA profiling, and molecular markers.
 

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Contents

An Important Source of Variation in Eukaryotic Genomes
1
Chapter 2 Detecting DNA Variation by Molecular Markers
21
Chapter 3 Laboratory Equipment
75
Chapter 4 Methodology
81
Chapter 5 Evaluation of Molecular Marker Data
207
Chapter 6 Applications of DNA Fingerprinting in Plant Sciences
235
Chapter 7 Linkage Analysis and Genetic Maps
277
A Comparison
293
Appendix 1 Plant DNA Isolation Protocols
311
Appendix 2 Commercial Companies
323
Appendix 3 Computer Programs Dealing with the Evaluation of DNA Sequence Variation and Molecular Marker Data
329
Appendix 4 Web Pages of Interest
337
References
339
Index
427
Back cover
445
Copyright

SNiPs and Chips for DNA and RNA Profiling
301

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Popular passages

Page 362 - Feinberg AP and Vogelstein B. (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.
Page 417 - D. (1987) A sequence in M13 phage detects hypervariable minisatellites in human and animal DNA. Science 235, 683-684.
Page 415 - Triglia, T., Peterson, MG, and Kemp, DJ (1988) A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res. 16, 8186.
Page 414 - TC 1994. Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. Theoretical and Applied Genetics, 88: 973-980.

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