Biochemical Applications of Raman and Resonance Raman SpectroscopesBiochemical Applications of Raman and Resonance Raman Spectroscopies focuses on the application of Raman and resonance Raman spectroscopies to biochemical problems. The book reviews biological systems and details the application of Raman spectroscopy to biological molecules such as proteins, nucleic acids, and lipids. It also looks at codevelopments of lasers, optics, and electronics that drive advances in experimental Raman spectroscopy, along with the important ramifications of these advances for biochemical applications. This volume is organized into eight chapters and begins with an overview of the theoretical and experimental aspects of Raman spectroscopy, including a very brief explanation of what Raman and resonance Raman spectroscopies are and a discussion of their advantages and disadvantages for biochemical studies. The explanation of the Raman and resonance Raman effects is taken up in more detail in the next chapter, which develops the concept of the vibrational motions of molecules by initially considering mechanical ""ball and spring"" models and goes on to use this concept to formulate a classical model for Raman scattering. The resonance Raman effect is then described by another model which emphasizes the discrete or quantized energy levels available to a molecule. The reader is also introduced to the experimental aspects of Raman spectroscopy and the application of Raman spectroscopy across the entire field of biochemistry. Each chapter contains an outline of the basic chemistry and biochemical nomenclature involved. This book will be of interest to chemists, biochemists, and spectroscopists, as well as graduate students and experienced research workers. |
Contents
| 1 | |
| 11 | |
CHAPTER 3 Experimental Raman Spectroscopy | 48 |
CHAPTER 4 Protein Conformation from Raman and Resonance Raman Spectra | 71 |
CHAPTER 5 Resonance Raman Studies of Natural ProteinBound Chromophores | 99 |
CHAPTER 6 Resonance Raman Labels | 154 |
CHAPTER 7 Nucleic Acids and Nucleic AcidProtein Complexes | 184 |
CHAPTER 8 Lipids Membranes and Carbohydrates | 208 |
Suggestions for Further Reading | 235 |
| 238 | |
| 253 | |
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absorption absorption spectrum Acta acyl enzyme all-trans amide amino acid aqueous assigned astaxanthin atoms attributable band base BChl binding Biochem Biochemistry biological Biophys Biopolymers Carey carotenoids cell changes Chem chemical chromophore co-workers complex components conformation containing depolarization electronic energy enzyme excitation profile Fe(III field first fluorescence frequency Gaber give rise hapten heme hydrogen bonds infrared intensity enhancement interactions Koenig laser beam ligand lipid Lippert membranes Mendelsohn molecular molecule monitor motion normal modes normal Raman nucleic acids observed obtained occurs optical oscillator peaks Peticolas phospholipid photons pigments polarized polypeptide porphyrin probe protein Raman intensity Raman scattering Raman spectra Raman Spectrosc Raman spectroscopy Raman studies resonance Raman rhodopsin RR spectra sample scattered light Section shift shown in Fig side chains solution spectrometer spectrum Spiro Strekas stretching region substrate temperature tion transition tRNA vibrational wavelength wavenumber X-ray