Somatic Cell HybridsThis book provides the background to the construction, characterization, and use of somatic cell hybrids. Particular attention is paid to their application in human gene mapping and in studies of gene expression, although other uses are also covered. The increasing utilization of somatic cell hybrids in biological research suggests there is a real need for a full description of how to make and use these cell lines and no other book covers this field as comprehensively. A number of techniques are clearly explained. This volume offers a useful introduction to the field for undergraduates, researchers at the beginning of their careers, and established scientists who wish to employ these techniques for the first time |
Contents
Introduction | 1 |
Southern blotting | 37 |
Modification of the basic techniquesdesigner hybrids | 52 |
Copyright | |
Common terms and phrases
Alu sequences Alu-PCR amplified analysis antibody biotinylated cDNA probe cell culture cell genetics cell hybrid DNA centromere Chapter chro chromo chromosome 11 chromosome content chromosome paint cloning CMGT complement dehydrogenase derived derX detect differentiated diploid disease gene example experiment fibroblasts Figure flasks fluorescent fused fusion gene mapping gene transfer genome grow hamster HAT medium HPRT HPRT gene human and rodent human cells human chromosome human DNA human gene mapping human/rodent hybrid cells hybrid containing hybrid panel IFGT intron isoenzymes karyotype laboratory loci locus malignancy mammalian cells mapping genes medium method Monochromosomal hybrids mosomes mouse chromosomes mouse/hamster mutations normal human original panel of somatic parental cell lines particular chromosome PCR primers PCR product permanent cell lines polymorphism possible problem protein pseudogenes region retained rodent rodent cell rodent chromosomes rodent DNA somatic cell hybrids Southern blotting species technique telomere tion tumour usually want to map X chromosome