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Measurement of Pyrimidine Dimers by IonExchange
Direct Identification of Other Radiation Products in
Measurement of CrossLinks Formed by Treatment
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4NQO acid-soluble added adducts alkali-labile alkylated ammonium antibodies AP endonuclease AP sites Biochem Biol Biophys bovine serum albumin caffeine calf thymus Cancer centrifugation Chinese hamster chromatography coli column concentration containing counts counts per minute cross-link yield cultures damage denatured determined dialyzed digestion diluted dimer content DNA glycosylase DNA repair doses EDTA eluted endodeoxyribonuclease enzyme Escherichia coli ethanol excision extract filter fraction glycosylase guanine hydrolysis hydrolyzed incubated irradiated labeled lesions mammalian cells measured medium method methyl mg ml-1 MgCl2 mixture molecular weight molecule NaCl NaOH Natl neutral nicks nuclease nucleotides phage phosphate buffer photoreactivating PM2 DNA pmol precipitate prepared procedure protein purified pyrimidine dimers radiation radioactivity reaction removed room temperature sedimentation Setlow sodium solution solvent specific activity strand breaks substrate sucrose gradients sulfate supernatant T4 endonuclease thymine treated treatment Tris-HCl tubes UV-irradiated vitro washed