Enzymes of Secondary MetabolismPeter J. Lea The series, Methods in Plant Biochemistry, provides an authoritative reference on current techniques in the various fields of plant biochemical research. Each volume in the series will, under the expert guidance of a guest editor, deal with a particular group of plant compounds. Each will describe the historical background and current, most useful methods of analysis. The volumes include detailed discussions of the protocols and suitability of each technique. Case treatments, diagrams, chemical structures, reference data, and properties will be featured along with a full list of references to the specialist literature. Conceived as a practical companion to The Biochemistry of Plants, edited by P.K. Stumpf and E.E. Conn, no plant biochemical laboratory can afford to be without this comprehensive and up-to-date reference source. |
Contents
Introduction | 26 |
Hydrolysable Tannins | 28 |
Enzyme assay techniques | 35 |
Copyright | |
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2-mercaptoethanol Academic Press acetate acetone alkaloids amino acid ammonium sulphate Arch ascorbate assay Biochem Biochemistry Biol Biophys biosynthesis carotenoids catalyses centrifuged chalcone Chem chemical chlorophyll chloroplasts chromatography cofactor column compounds concentration Conn containing COOH Croteau crude extract cyanogenic glycosides cyanohydrin cyclase Cysteine synthase cytochrome P-450 EDTA eluted enzymatic enzyme activity enzyme preparation ester flavonoid Forkmann formation fractions glucose glucosinolate Grisebach Halkier higher plants homogeneity homogenised Hösel HPLC hydroxylase hydroxylation Ikegami incubation inhibition inhibitors intermediates Isoenzyme isolated lyase membrane method methyl microsomal molecular weight Møller monoterpene Murakoshi myrosinase NADPH Naturforsch nmol oxidase oxidation partially purified pathway phosphate buffer pH Phytochemistry phytoene Plant Physiol plastid Poulton precipitate prenyltransferases procedure protein purification pyrophosphate radioactivity reaction mixture reagent reductase saturation seedlings Sephadex sodium specific activity Strack substrate supernatant synthesis tissue Tris-HCl buffer Underhill urogen Zenk µmol