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PLASMIDS AND TRANSPOSONS
IncFII Plasmid Incompatibility Product and Its Target Are Both RNA Tran
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activity added addition amino acid analysis assay B-galactosidase Bacillus bacterial Bacteriol bacteriophage Biochem Biol buffer carrying cells changes chromosome cloned complex concentration constructed containing copy culture cytoplasmic deletion derivatives described detected determined digested EcoRI effect enzyme Escherichia coli experiments expression extracts followed fragment function fusion gene genetic glucose grown growth Harbor hybridization increase incubation indicated induced inhibition initial insertion isolated lane located measured medium membrane method Microbiol molecular mutants observed obtained operon phage phosphate plasmid plates position prepared presence previously proline promoter protein purified recA recombinant region regulation replication reported resistance restriction samples selected sequence showed shown similar sodium specific spiroplasmas strain structure subtilis suggest synthesis Table tested tion trans transcription transfer transformation transport uptake washed whereas